DR MRITTIKA SEN

Dr. SANTOSH G. HONAVAR, Dr. KAUSTUBH MULAY, Dr.Shivashankar T

 

Abstract

Pathology plays a very important role in the management of various ocular lesions. Biopsy of ocular and orbital tumours are performed for diagnosis, establishing the tumour margins, identifying high risk features, for the prognosis of the patient and to determine the need for adjuvant therapy. Ophthalmic biopsies can be of various types- excisional, incisional, punch, shave, multilevel, map and frozen sections. In this video we discuss about the specific indications of each of them and the various stages of processing through which a biopsy specimen passes, before it reaches the pathologist, including transportation and preservation, gross examination, pathological sectioning of the tissue, fixation in formalin, preparation of tissue blocks in paraffin, tissue slicing and preparation of slides, staining of slides with specific stains and quality control. Immunohistochemistry has additional role in the final diagnosis and management which will also be touched upon.